The ability of the BANA test to detect different levels of P. gingivalis, T. denticola and T. forsythia

The aim of this study was to evaluate the ability of the BANA Test to detect different levels of Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia or their combinations in subgingival samples at the initial diagnosis and after periodontal therapy. Periodontal sites with probing depths between 5-7 mm and clinical attachment level between 5-10 mm, from 53 subjects with chronic periodontitis, were sampled in four periods: initial diagnosis (T0), immediately (T1), 45 (T2) and 60 days (T3) after scaling and root planing. BANA Test and Checkerboard DNA-DNA hybridization identified red complex species in the subgingival biofilm. In all experimental periods, the highest frequencies of score 2 (Checkerboard DNA-DNA hybridization) for P. gingivalis, T. denticola and T. forsythia were observed when strong enzymatic activity (BANA) was present (p < 0.01). The best agreement was observed at initial diagnosis. The BANA Test sensitivity was 95.54% (T0), 65.18% (T1), 65.22% (T2) and 50.26% (T3). The specificity values were 12.24% (T0), 57.38% (T1), 46.27% (T2) and 53.48% (T3). The BANA Test is more effective for the detection of red complex pathogens when the bacterial levels are high, i.e. in the initial diagnosis of chronic periodontitis

Predictive Stator Flux and Load Angle Control of Synchronous Reluctance Motor Drives Operating in a Wide Speed Range

This paper presents a new simplified finitecontrol- set model predictive control strategy for synchronous reluctance motors operating in the entire speed range. It is a predictive control scheme that regulates the stator flux and the load angle of the synchronous reluctance motor, incorporating the ability to operate the drive in the field-weakening region and respecting the motor voltage and current limits as well as the load angle limitation needed to operate this type of motor in the maximum torque per voltage region. The proposed control strategy possesses some attractive features, such as no need for controller calibration, no weighting factors in the cost function, good robustness against parameter mismatch, and smaller computational cost compared to more traditional finite-control-set model predictive control algorithms. Simulation and experimental results obtained using a high-efficiency synchronous reluctance motor demonstrate the effectiveness of the proposed control scheme.info:eu-repo/semantics/publishedVersio

DIFFERENT THERAPEUTIC METHODS RELATED TO PERI-IMPLANTITIS: AN UMBRELLA REVIEW

As doenças peri-implantares são definidas como lesões inflamatórias dos tecidos que circundam os implantes dentários, abrangendo a mucosite peri-implantar e a peri-implantite. O objetivo deste trabalho foi avaliar as revisões sistemáticas publicadas em periódicos indexados sobre os diversos tipos de tratamento para a peri-implantite. Uma visão geral de revisões sistemáticas (Umbrella Review) foi realizada através da avaliação de periódicos publicados em banco de dados eletrônicos (PubMed, Cochrane, MEDLINE, LILACS, Science Direct), utilizando os descritores: Peri-implantite OU Doença Peri-implantar OU Infecção peri-implantar E Tratamento peri-implantar OU Terapia peri-implantar, sem restrição de idiomas, entre 2011 e 2022. Também foi realizada uma estratégia de busca manual específica nas referências dos artigos selecionados. Quarenta e oito artigos foram encontrados, destes, 34 artigos foram eleitos para compor esse estudo. Foram excluídos relatos de casos, estudos observacionais, ensaios clínicos randomizados ou não randomizados, estudos experimentais, comentários, resumos expandidos, e estudos em duplicata. A terapia peri-implantar não cirúrgica envolve o debridamento mecânico da superfície do implante usando curetas, dispositivos ultrassônicos ou abrasivos a ar ou lasers, com ou sem o uso adjuvante de antibióticos ou antissépticos locais. A eficácia das diversas terapias peri-implantares mostrou‐se limitada, inclusive para a associação de antimicrobianos, especialmente em termos de redução da profundidade de sondagem e ganho de tecido ósseo. Dessa forma, sugere-se a necessidade da realização de novas revisões sistemáticas com melhor delineamento metodológico e mínimo de vieses. Assim, resultados mais previsíveis serão alcançados e promoverão uma maior confiança na escolha da forma terapêutica ideal para cada situação clínica.Peri-implant diseases are defined as inflammatory lesions of the tissues surrounding dental implants, including peri-implant mucositis and peri-implantitis. The aim of this study was to evaluate the systematic reviews published in indexed journals on the different types of treatment for peri-implantitis. An Umbrella Review was performed by evaluating journals published in electronic databases (PubMed, Cochrane, MEDLINE, LILACS, Science Direct), using the descriptors: Peri-implantitis OR Peri-implant disease OR Infection peri-implant AND peri-implant treatment OR peri-implant therapy, without language restriction, between 2011 and 2022. A specific manual search strategy was also performed in the references of the selected articles. Forty-eight articles were found, of these, 34 articles were chosen to compose this study. Case reports, observational studies, randomized or non-randomized clinical trials, experimental studies, reviews, expanded abstracts, and duplicate studies were excluded. Nonsurgical peri-implant therapy involves mechanical debridement of the implant surface using curettes, ultrasonic or air abrasive devices, or lasers, with or without the adjunctive use of antibiotics or local antiseptics. The effectiveness of the various peri-implant therapies has been shown to be limited, including the combination of antimicrobials, especially in terms of reduced probing depth and bone tissue gain. Thus, it is suggested the need to carry out new systematic reviews with better methodological design and minimal bias. Thus, more predictable results will be achieved and will promote greater confidence in choosing the ideal therapeutic form for each clinical situation.As doenças peri-implantares são definidas como lesões inflamatórias dos tecidos que circundam os implantes dentários, abrangendo a mucosite peri-implantar e a peri-implantite. O objetivo deste trabalho foi avaliar as revisões sistemáticas publicadas em periódicos indexados sobre os diversos tipos de tratamento para a peri-implantite. Uma visão geral de revisões sistemáticas (Umbrella Review) foi realizada através da avaliação de periódicos publicados em banco de dados eletrônicos (PubMed, Cochrane, MEDLINE, LILACS, Science Direct), utilizando os descritores: Peri-implantite OU Doença Peri-implantar OU Infecção peri-implantar E Tratamento peri-implantar OU Terapia peri-implantar, sem restrição de idiomas, entre 2011 e 2022. Também foi realizada uma estratégia de busca manual específica nas referências dos artigos selecionados. Quarenta e oito artigos foram encontrados, destes, 34 artigos foram eleitos para compor esse estudo. Foram excluídos relatos de casos, estudos observacionais, ensaios clínicos randomizados ou não randomizados, estudos experimentais, comentários, resumos expandidos, e estudos em duplicata. A terapia peri-implantar não cirúrgica envolve o debridamento mecânico da superfície do implante usando curetas, dispositivos ultrassônicos ou abrasivos a ar ou lasers, com ou sem o uso adjuvante de antibióticos ou antissépticos locais. A eficácia das diversas terapias peri-implantares mostrou‐se limitada, inclusive para a associação de antimicrobianos, especialmente em termos de redução da profundidade de sondagem e ganho de tecido ósseo. Dessa forma, sugere-se a necessidade da realização de novas revisões sistemáticas com melhor delineamento metodológico e mínimo de vieses. Assim, resultados mais previsíveis serão alcançados e promoverão uma maior confiança na escolha da forma terapêutica ideal para cada situação clínica.Las enfermedades periimplantarias se definen como lesiones inflamatorias de los tejidos que rodean los implantes dentales, que abarcan la mucositis periimplantaria y la periimplantitis. El objetivo de este estudio fue evaluar las revisiones sistemáticas publicadas en revistas indexadas sobre los diversos tipos de tratamiento para la periimplantitis. Se realizó una visión general de las revisiones sistemáticas (Umbrella Review) a través de la evaluación de revistas publicadas en bases de datos electrónicas (PubMed, Cochrane, MEDLINE, LILACS, Science Direct), utilizando los descriptores: Periimplantitis O enfermedad periimplantaria O infección periimplantaria Y Tratamiento periimplantario O terapia periimplantaria, sin restricción de idioma, entre 2011 y 2022. También se realizó una estrategia específica de búsqueda manual en las referencias de los artículos seleccionados. Se encontraron cuarenta y ocho artículos, de estos, 34 artículos fueron elegidos para componer este estudio. Se excluyeron los informes de casos, los estudios observacionales, los ensayos clínicos aleatorizados o no aleatorizados, los estudios experimentales, los comentarios, los resúmenes ampliados y los estudios duplicados. La terapia periimplantaria no quirúrgica implica el desbridamiento mecánico de la superficie del implante utilizando curetas, dispositivos de aire ultrasónicos o abrasivos, o láseres, con o sin el uso adyuvante de antibióticos o antisépticos locales. La eficacia de las diversas terapias periimplantarias fue limitada, incluso para la combinación de antimicrobianos, especialmente en términos de reducción de la profundidad del sondeo y obtención de tejido óseo. Por lo tanto, se sugiere la necesidad de nuevas revisiones sistemáticas con mejor diseño metodológico y sesgo mínimo.

Successful and failed mini-implants: microbiological evaluation and quantification of bacterial endotoxin

Objectives: Using two groups of mini-implants (successful and failed) the objectives of this in vivo study were: to evaluate the microbial contamination by the checkerboard DNA-DNA hybridization technique and to quantify the bacterial endotoxin by the limulus amebocyte lysate assay. Material and Methods:The 15 successful and 10 failed mini-implants (1.6 mm diameter × 7.0 or 9.0 mm long), placed in the maxilla and/or mandible, were obtained from 15 patients undergoing orthodontic treatment. Data were analyzed statistically by the Wilcoxon rank-sum test using the SAS software (a=0.05). Results: All 40 microbial species were detected in both groups of mini-implants, with different frequencies. No differences were observed between the groups with respect to microbial complexes (blue, purple, yellow, green, orange, red and other species) and endotoxin quantification (p&gt;0.05). Conclusion: Neither microbial contamination nor endotoxin quantification was determinant for the early loss of stability of the mini-implant

Molecular detection of in-vivo microbial contamination of metallic orthodontic brackets by checkerboard DNA-DNA hybridization

Introduction: Knowing the microbiota that colonizes orthodontic appliances is important for planning strategies and implementing specific preventive measures during treatment. The purpose of this clinical trial was to evaluate in vivo the contamination of metallic orthodontic brackets with 40 DNA probes for different bacterial species by using the checkerboard DNA-DNA hybridization (CDDH) technique. Methods: Eighteen patients, 11 to 29 years of age having fixed orthodontic treatment, were enrolled in the study. Each subject had 2 new metallic brackets bonded to different premolars in a randomized manner. After 30 days, the brackets were removed and processed for analysis by CDDH. Data on bacterial contamination were analyzed descriptively and with the Kruskal-Wallis and Dunn post tests (a 5 0.05). Forty microbial species (cariogenic microorganisms, bacteria of the purple, yellow, green, orange complexes, &quot;red complex 1 Treponema socranskii,&quot; and the cluster of Actinomyces) were assessed. Results: Most bacterial species were present in all subjects, except for Streptococcus constellatus, Campylobacter rectus, Tannerella forsythia, T socranskii, and Lactobacillus acidophillus (94.4%), Propionibacterium acnes I and Eubacterium nodatum (88.9%), and Treponema denticola (77.8%). Among the cariogenic microorganisms, Streptococcus mutans and Streptococcus sobrinus were found in larger numbers than L acidophillus and Lactobacillus casei (P \0.001). The periodontal pathogens of the orange complex were detected in larger numbers than those of the &quot;red complex 1 T socranskii&quot; (P \0.0001). Among the bacteria not associated with specific pathologies, Veillonella parvula (purple complex) was the most frequently detected strain (P \0.0001). The numbers of yellow and green complex bacteria and the cluster of Actinomyces were similar (P .0.05). Conclusions: Metallic brackets in use for 1 month were multi-colonized by several bacterial species, including cariogenic microorganisms and periodontal pathogens, reinforcing the need for meticulous oral hygiene and additional preventive measures to maintain oral health in orthodontic patients. (Am J Orthod Dentofacial Orthop 2012;141:24-9

Changes in the subgingival biofilm composition after coronally positioned flap

OBJECTIVES: This study evaluated the effects of coronally positioned flap (CPF) on the subgingival biofilm composition. MATERIAL AND METHODS: Twenty-two subjects with gingival recessions were treated with CPF. Clinical parameters were assessed before and at 6 months after surgery. Subgingival biofilms were analyzed by checkerboard DNA-DNA hybridization technique for 40 bacterial species. RESULTS: Recession height, clinical attachment level and bleeding on probing improved significantly (

Microbiological composition associated with vitamin D receptor gene polymorphism in chronic periodontitis

The aim of this cross-sectional study was to examine the relationship between the composition of the subgingival microbiota and the vitamin D receptor (VDR) gene polymorphism in Brazilian adults with chronic periodontitis. The clinical parameters of probing depth, clinical attachment level, bleeding on probing, plaque accumulation and suppuration were measured in 60 Caucasian adults who were divided into two groups: 30 healthy individuals (control) and 30 with chronic periodontitis (ChP). Subgingival plaque samples were collected from 6 sites per subject and analyzed for 38 bacterial species using the Checkerboard DNA-DNA Hybridization. DNA was obtained from the subjects' epithelial cells by scraping the buccal mucosa and using a mouthwash containing 3% of glucose. Polymorphism in the VDR gene was analyzed by the polymerase chain reaction (PCR), followed by Taql digestion (RFLP). The healthy subjects presented significantly lower levels (0.3 &#215; 10(7) ± 0.7 &#215; 10(7)) of total microbial counts in comparison with subjects with chronic periodontitis (4.5 &#215; 10(7) ± 2.9 &#215; 10(7)). Regarding the occurrence of VDR polymorphism, it was observed that the Tt genotype was more prevalent in the Periodontitis group (60%) than in the Healthy group (30%), while the prevalences of the TT genotype were 23.3% and 53.3%, respectively (Chi-square test, p < 0.05). No difference was found in the composition of subgingival microbiota among the VDR genotypes evaluated for the Healthy and Periodontitis groups. In conclusion, the Tt genotype was associated with periodontal disease; however, no association with the subgingival microbiota was observed

Influence of implant surface topography on early osseointegration: A histological study in human jaws

The purpose of this study was to evaluate the influence of the oxidized surface on bone-to-implant contact (BIC%), the bone density in the threaded area (BA %), as well as the bone density outside the threaded area (BD%) in human jaws after 2 months of unloaded healing. Thirteen subjects (mean age 42.61 +/- 6.15 years) received two microimplants (2.5 mm diameter and 6 mm length) each, during conventional mandible or maxilla implant surgery. The microimplants with commercially pure titanium surfaces (machined) and oxidized surfaces served as the control and test surfaces, respectively. After 2 months, the microimplants and the surrounding tissue were removed and prepared for histomorphometric analysis. All microimplants, except two machined and one oxidized microimplant surfaces, were found to be clinically stable after the healing period. Histometric evaluation indicated that the mean BIC % was (21.71 +/- 13.11) % and (39.04 +/- 15.75) % for machined and oxidized microimplant surfaces, respectively. The BD% was higher for the oxidized surface, although there was no difference for maxilla and mandible. The oxidized surface impacted the BA% for the type-IV bone. Data suggest that the oxidized surface presented a higher bone-to-implant contact rate compared with machined surfaces under unloaded conditions, after a healing period of 2 months. (c) 2006 Wiley Periodicals, Inc